MALDI-TOF mass spectrometry and identification of new bacteria species in air samples from Makkah, Saudi Arabia.

ABSTRACT:

Background

During the Hajj season, respiratory symptoms are very common among pilgrims. Here, we investigated the viable bacterial population in air samples collected around the slaughterhouses used during the Hajj.

Methods and results

We collected air samples on three days from four different sites: slaughterhouses at Al-Kakia, Al-Meaisim and Al-Sharaia, and from a waste disposal area designated for the remnants of slaughter. Samples were cultured on blood agar plates for 48 h, and bacterial isolates were identified using MALDI-TOF MS. A dendrogram using the spectra of the unidentified bacterial species was constructed, and PCR amplification and sequencing of the 16S rRNA gene was performed for one isolate per cluster. In total, 2500 colonies appeared on the nutrient agar p

SUBMITTER: Angelakis E 

PROVIDER: S-EPMC4295573 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature