Reprogramming of Yersinia from virulent to persistent mode revealed by complex in vivo RNA-seq analysis.

ABSTRACT: We recently found that Yersinia pseudotuberculosis can be used as a model of persistent bacterial infections. We performed in vivo RNA-seq of bacteria in small cecal tissue biopsies at early and persistent stages of infection to determine strategies associated with persistence. Comprehensive analysis of mixed RNA populations from infected tissues revealed that Y. pseudotuberculosis undergoes transcriptional reprogramming with drastic down-regulation of T3SS virulence genes during persistence when the pathogen resides within the cecum. At the persistent stage, the expression pattern in many respects resembles the pattern seen in vitro at 26oC, with for example, up-regulation of flagellar genes and invA. These findings are expected to have impact on future rationales to identify suitable bac

SUBMITTER: Avican K 

PROVIDER: S-EPMC4295882 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature