Microarray Analysis of Defective Cartilage in Hoxc8- and Hoxd4-Transgenic Mice.

ABSTRACT:

Objective

Homeobox genes of the Hox class are required for proper patterning of skeletal elements and play a role in cartilage differentiation. In transgenic mice with overexpression of Hoxc8 and Hoxd4 during cartilage development, the authors observed severe defects, namely, physical instability of cartilage, accumulation of immature chondrocytes, and decreased maturation to hypertrophy. To define the molecular basis underlying these defects, the authors performed gene expression profiling using the Affymetrix microarray platform.

Results

Primary chondrocytes were isolated from Hoxc8- and Hoxd4-transgenic mouse embryo rib cartilage at 18.5 days of gestation. In both cases, differentially expressed genes were identified that have a role in cell proliferation and cell cycle r

SUBMITTER: Kruger C 

PROVIDER: S-EPMC4297070 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature