Dataset Information

Cyclooxygenase-1 as the main source of proinflammatory factors after sodium orthovanadate treatment.

ABSTRACT: Vanadium is a metal present in air pollution. Its compounds may have both anticancer and carcinogenic properties. Vanadium compounds are tested in treatment of diabetes and cancer. An important research direction aimed at better understanding of the mechanisms of action of the vanadium compounds is a more detailed insight into their impact on inflammatory reactions. The aim of this study was to examine the effect of micromolar concentrations of sodium orthovanadate, Na3VO4, on the activity and expression of cyclooxygenases: COX-1 and COX-2. PMA-activated THP-1 macrophages were incubated in vitro for 48 h with micromolar concentrations of sodium orthovanadate. As shown by an ELISA assay, sodium orthovanadate increases the quantity of prostaglandin E2 being released into the medium in a dose-dependent manner as well as impacts the quantity of the stable metabolite of thromboxane A2: thromboxane B2. The use of a COX-2 inhibitor, NS-398, revealed that this effect was independent of changes in the activity of COX-2. Western blotting analysis showed that sodium orthovanadate increased the expression of COX-2 when used with NS-398. Quantitative real-time PCR measurements of mRNA levels of genes PTGS1 and PTGS2 revealed no effect of the tested vanadium compound on the levels of analyzed transcripts.

SUBMITTER: Korbecki J

PROVIDER: S-EPMC4297299 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Cyclooxygenase-1 as the main source of proinflammatory factors after sodium orthovanadate treatment.

Korbecki Jan J Baranowska-Bosiacka Irena I Gutowska Izabela I Piotrowska Katarzyna K Chlubek Dariusz D

Biological trace element research 20141115 1-2

Vanadium is a metal present in air pollution. Its compounds may have both anticancer and carcinogenic properties. Vanadium compounds are tested in treatment of diabetes and cancer. An important research direction aimed at better understanding of the mechanisms of action of the vanadium compounds is a more detailed insight into their impact on inflammatory reactions. The aim of this study was to examine the effect of micromolar concentrations of sodium orthovanadate, Na3VO4, on the activity and e ...[more]

PMID: 25398544

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Project description:The Beijing-Tianjin Sandstorm Source Region (BTSSR), a region with significant vegetation degradation in China, has been subjected to ecological engineering intended to curb vegetation browning. Nevertheless, few studies have used multisource data to quantitatively evaluate the vegetation restoration effectiveness in the BTSSR, and the relationship between ecological engineering and vegetation restoration effectiveness in this region from statistical evidence has received little attention so far. Here, we employed the comprehensive vegetation parameters to describe the vegetation restoration effectiveness, and examined the driving mechanism of natural and human factors in different sub regions. First, we evaluated the vegetation restoration effectiveness in the BTSSR using an index that combined Fractional Vegetation Coverage (FVC) and Net Primary Productivity (NPP). Our results showed that the vegetation restoration effectiveness has significantly increased over time. From 2000 to 2020, 60.9% of the area achieved significant vegetation restoration, and the area with higher vegetation restoration effectiveness was concentrated in the southern part of the study area. Then, we used the Geodetector Model to explore the main factors and their interactions affecting vegetation restoration effectiveness. We found that the vegetation restoration effectiveness in the entire area was dominated by annual precipitation, in the northern part of the study area was led by climate, and in the southern part of the study area was dominated by ecological engineering. We further demonstrated that the interaction between ecological engineering and climate, soil conditions, geographical background and socioeconomic had the synergistic effect on vegetation restoration effectiveness, and the interaction between ecological engineering and annual precipitation had the greatest impact. We recommend that the northern region of the BTSSR continue to build low-density wind and sand control forests, while the southern region needs to be strengthened to prevent soil erosion problems caused by the expansion of human activities.

Dataset Information

Cyclooxygenase-1 as the main source of proinflammatory factors after sodium orthovanadate treatment.

Publications

Cyclooxygenase-1 as the main source of proinflammatory factors after sodium orthovanadate treatment.

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