Isolation and enrichment of mouse female germ line stem cells.

ABSTRACT: OBJECTIVE:The existence of female germ-line stem cells (FGSCs) has been the subject of a wide range of recent studies. Successful isolation and culture of FGSCs could facilitate studies on regenerative medicine and infertility treatments in the near future. Our aim in the present study was evaluation of the most commonly used techniques in enrichment of FGSCs and in establishment of the best procedure. MATERIALS AND METHODS:In this experimental study, after digesting neonate ovary from C57Bl/6 mice, we performed 2 different isolation experiments: magnetic activated cell sorting (MACS) and pre-plating. MACS was applied using two different antibodies against mouse vasa homolog (MVH) and stage-specific embryonic antigen-1 (SSEA1) markers. After the cells were passaged and proliferated in vitr

SUBMITTER: Khosravi-Farsani S 

PROVIDER: S-EPMC4297479 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature