Comparison of DNA methylation profiles in human fetal and adult red blood cell progenitors.

ABSTRACT:

Background

DNA methylation is an epigenetic modification that plays an important role during mammalian development. Around birth in humans, the main site of red blood cell production moves from the fetal liver to the bone marrow. DNA methylation changes at the β-globin locus and a switch from fetal to adult hemoglobin production characterize this transition. Understanding this globin switch may improve the treatment of patients with sickle cell disease and β-thalassemia, two of the most common Mendelian diseases in the world. The goal of our study was to describe and compare the genome-wide patterns of DNA methylation in fetal and adult human erythroblasts.

Methods

We used the Illumina HumanMethylation 450 k BeadChip to measure DNA methylation at 402,819 CpGs in ex vivo-diff

SUBMITTER: Lessard S 

PROVIDER: S-EPMC4298057 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature