Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites.

ABSTRACT:

Background

To investigate the microbial composition of biofilms at inflamed peri-implant and periodontal tissues in the same subject, using 16S rRNA sequencing.

Methods

Supra- and submucosal, and supra- and subgingival plaque samples were collected from 7 subjects suffering from diseased peri-implant and periodontal tissues. Bacterial DNA was isolated and 16S rRNA genes were amplified, sequenced and aligned for the identification of bacterial genera.

Results

43734 chimera-depleted, denoised sequences were identified, corresponding to 1 phylum, 8 classes, 10 orders, 44 families and 150 genera. The most abundant families or genera found in supramucosal or supragingival plaque were Streptoccocaceae, Rothia and Porphyromonas. In submucosal plaque, the most abundant family

SUBMITTER: Schaumann S 

PROVIDER: S-EPMC4298060 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature