Genome-wide chromatin footprinting reveals changes in replication origin architecture induced by pre-RC assembly.

ABSTRACT: Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replication origins. To reveal how the chromatin architecture modulates origin selection and activation, we "footprinted" nucleosomes, transcription factors, and replication proteins at multiple points during the Saccharomyces cerevisiae cell cycle. Our nucleotide-resolution protein occupancy profiles resolved a precise ORC-dependent footprint at 269 origins in G2. A separate class of inefficient origins exhibited protein occupancy only in G1, suggesting that stable ORC chromatin asso

SUBMITTER: Belsky JA 

PROVIDER: S-EPMC4298139 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature