Anchoring and ordering NGS contig assemblies by population sequencing (POPSEQ).

ABSTRACT: Next-generation whole-genome shotgun assemblies of complex genomes are highly useful, but fail to link nearby sequence contigs with each other or provide a linear order of contigs along individual chromosomes. Here, we introduce a strategy based on sequencing progeny of a segregating population that allows de novo production of a genetically anchored linear assembly of the gene space of an organism. We demonstrate the power of the approach by reconstructing the chromosomal organization of the gene space of barley, a large, complex and highly repetitive 5.1 Gb genome. We evaluate the robustness of the new assembly by comparison to a recently released physical and genetic framework of the barley genome, and to various genetically ordered sequence-based genotypic datasets. The method is indep

SUBMITTER: Mascher M 

PROVIDER: S-EPMC4298792 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature