Controlled analysis of nanoparticle charge on mucosal and systemic antibody responses following pulmonary immunization.

ABSTRACT: Pulmonary immunization enhances local humoral and cell-mediated mucosal protection, which are critical for vaccination against lung-specific pathogens such as influenza or tuberculosis. A variety of nanoparticle (NP) formulations have been tested preclinically for pulmonary vaccine development, yet the role of NP surface charge on downstream immune responses remains poorly understood. We used the Particle Replication in Non-Wetting Templates (PRINT) process to synthesize hydrogel NPs that varied only in surface charge and otherwise maintained constant size, shape, and antigen loading. Pulmonary immunization with ovalbumin (OVA)-conjugated cationic NPs led to enhanced systemic and lung antibody titers compared with anionic NPs. Increased antibody production correlated with robust germinal c

SUBMITTER: Fromen CA 

PROVIDER: S-EPMC4299250 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature