NOB1 is essential for the survival of RKO colorectal cancer cells.

ABSTRACT:

Aim

To determine the role of NOB1, a regulator of cell survival in yeast, in human colorectal cancer cells.

Methods

Lentivirus-mediated small interfering RNA (siRNA) was used to inhibit NOB1 expression in RKO human colorectal cancer cells in vitro and in vivo in a mouse xenograft model. The in vitro and in vivo knockdown efficacy was determined using both Western blot and quantitative reverse transcription polymerase chain reaction (qRT-PCR). qRT-PCR was also used to analyze the downstream signals following NOB1 knockdown. Cell growth and colony formation assays were used to determine the effect of NOB1 inhibition on RKO proliferation and their ability to form colonies. Endonuclease activity, as evaluated by terminal deoxytransferase-mediated dUTP nick end labeling (TUNEL),

SUBMITTER: He XW 

PROVIDER: S-EPMC4299339 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature