MicroRNA miR145 regulates TGFBR2 expression and matrix synthesis in vascular smooth muscle cells.

ABSTRACT:

Rationale

MicroRNA miR145 has been implicated in vascular smooth muscle cell differentiation, but its mechanisms of action and downstream targets have not been fully defined.

Objective

Here, we sought to explore and define the mechanisms of miR145 function in smooth muscle cells.

Methods and results

Using a combination of cell culture assays and in vivo mouse models to modulate miR145, we characterized its downstream actions on smooth muscle phenotypes. Our results show that the miR-143/145 gene cluster is induced in smooth muscle cells by coculture with endothelial cells. Endothelial cell-induced expression of miR-143/145 is augmented by Notch signaling and accordingly expression is reduced in Notch receptor-deficient cells. Screens to identify miR145-regulated genes

SUBMITTER: Zhao N 

PROVIDER: S-EPMC4299754 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature