Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli.

ABSTRACT:

Background

Recombinant protein-based therapeutics have become indispensable for the treatment of many diseases. They are produced using well-established expression systems based on bacteria, yeast, insect and mammalian cells. The majority of therapeutic proteins are glycoproteins and therefore the post-translational attachment of sugar residues is required. The development of an engineered Escherichia coli-based expression system for production of human glycoproteins could potentially lead to increased yields, as well as significant decreases in processing time and costs.

Results

This work describes the expression of functional human-derived glycosyltransferase UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAcT2) in a recombinant E. coli strain. For expressi

SUBMITTER: Lauber J 

PROVIDER: S-EPMC4299809 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature