Prevalent and distinct spliceosomal 3'-end processing mechanisms for fungal telomerase RNA.

ABSTRACT: Telomerase RNA (TER) is an essential component of the telomerase ribonucleoprotein complex. The mechanism for TER 3'-end processing is highly divergent among different organisms. Here we report a unique spliceosome-mediated TER 3'-end cleavage mechanism in Neurospora crassa that is distinct from that found specifically in the fission yeast Schizosaccharomyces pombe. While the S. pombe TER intron contains the canonical 5'-splice site GUAUGU, the N. crassa TER intron contains a non-canonical 5'-splice site AUAAGU that alone prevents the second step of splicing and promotes spliceosomal cleavage. The unique N. crassa TER 5'-splice site sequence is evolutionarily conserved in TERs from Pezizomycotina and early branching Taphrinomycotina species. This suggests that the widespread and basal N. c

SUBMITTER: Qi X 

PROVIDER: S-EPMC4299825 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature