The meta-epigenomic structure of purified human stem cell populations is defined at cis-regulatory sequences.

ABSTRACT: The mechanism and significance of epigenetic variability in the same cell type between healthy individuals are not clear. Here we purify human CD34+ haematopoietic stem and progenitor cells (HSPCs) from different individuals and find that there is increased variability of DNA methylation at loci with properties of promoters and enhancers. The variability is especially enriched at candidate enhancers near genes transitioning between silent and expressed states, and encoding proteins with leukocyte differentiation properties. Our findings of increased variability at loci with intermediate DNA methylation values, at candidate 'poised' enhancers and at genes involved in HSPC lineage commitment suggest that CD34+ cell subtype heterogeneity between individuals is a major mechanism for the variab

SUBMITTER: Wijetunga NA 

PROVIDER: S-EPMC4300104 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature