High-throughput profiling of point mutations across the HIV-1 genome.

ABSTRACT:

Background

The HIV-1 pandemic is not the result of a static pathogen but a large genetically diverse and dynamic viral population. The virus is characterized by a highly mutable genome rendering efforts to design a universal vaccine a significant challenge and drives the emergence of drug resistant variants upon antiviral pressure. Gaining a comprehensive understanding of the mutational tolerance of each HIV-1 genomic position is therefore of critical importance.

Results

Here we combine high-density mutagenesis with the power of next-generation sequencing to gauge the replication capacity and therefore mutational tolerability of single point mutations across the entire HIV-1 genome. We were able to achieve the evaluation of point mutational effects on viral replicative capac

SUBMITTER: Al-Mawsawi LQ 

PROVIDER: S-EPMC4300175 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature