Less is more: Ebola virus surface glycoprotein expression levels regulate virus production and infectivity.

ABSTRACT:

Unlabelled

The Ebola virus (EBOV) surface glycoprotein (GP1,2) mediates host cell attachment and fusion and is the primary target for host neutralizing antibodies. Expression of GP1,2 at high levels disrupts normal cell physiology, and EBOV uses an RNA-editing mechanism to regulate expression of the GP gene. In this study, we demonstrate that high levels of GP1,2 expression impair production and release of EBOV virus-like particles (VLPs) as well as infectivity of GP1,2-pseudotyped viruses. We further show that this effect is mediated through two mechanisms. First, high levels of GP1,2 expression reduce synthesis of other proteins needed for virus assembly. Second, viruses containing high levels of GP1,2 are intrinsically less infectious, possibly due to impaired receptor binding o

SUBMITTER: Mohan GS 

PROVIDER: S-EPMC4300637 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature