Mapping mutations in plant genomes with the user-friendly web application CandiSNP.

ABSTRACT:

Background

Analysis of mutants isolated from forward-genetic screens has revealed key components of several plant signalling pathways. Mapping mutations by position, either using classical methods or whole genome high-throughput sequencing (HTS), largely relies on the analysis of genome-wide polymorphisms in F2 recombinant populations. Combining bulk segregant analysis with HTS has accelerated the identification of causative mutations and has been widely adopted in many research programmes. A major advantage of HTS is the ability to perform bulk segregant analysis after back-crossing to the parental line rather than out-crossing to a polymorphic ecotype, which reduces genetic complexity and avoids issues with phenotype penetrance in different ecotypes. Plotting the positions of hom

SUBMITTER: Etherington GJ 

PROVIDER: S-EPMC4301057 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature