Transcriptional profiling reveals functional links between RasGrf1 and Pttg1 in pancreatic beta cells.

ABSTRACT:

Background

Our prior characterization of RasGrf1 deficient mice uncovered significant defects in pancreatic islet count and size as well as beta cell development and signaling function, raising question about the mechanisms linking RasGrf1 to the generation of those "pancreatic" phenotypes.

Results

Here, we compared the transcriptional profile of highly purified pancreatic islets from RasGrf1 KO mice to that of WT control animals using commercial oligonucleotide microarrays. RasGrf1 elimination resulted in differential gene expression of numerous components of MAPK- and Calcium-signaling pathways, suggesting a relevant contribution of this GEF to modulation of cellular signaling in the cell lineages integrating the pancreatic islets. Whereas the overall transcriptional profi

SUBMITTER: Manyes L 

PROVIDER: S-EPMC4301450 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature