Sonic hedgehog increases the skin wound-healing ability of mouse embryonic stem cells through the microRNA 200 family.

ABSTRACT:

Background and purpose

To use stem cell therapy effectively, it is important to enhance the therapeutic potential of stem cells with soluble factors. Although sonic hedgehog (shh) is important in maintaining the stem cell, the recovery effect of mouse embryonic stem cells (mESCs) with shh has not yet been elucidated. The present study investigated the effect of mESCs with shh in skin recovery in vivo as well as the related intracellular signal pathways in vitro.

Experimental approach

The healing effect of mESCs with shh on skin wounds was examined in vivo in ICR mice. The involvement of Smads, the microRNA (miR)-200 family, zinc finger E-box-binding homeobox (ZEBs) and E-cadherin on shh-induced mESC migration and self-renewal was determined in vitro.

Key results

The m

SUBMITTER: Suh HN 

PROVIDER: S-EPMC4301691 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature