The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer.

ABSTRACT:

Background

Regulatory T cells (Treg) are enriched in human colorectal cancer (CRC) where they suppress anti-tumour immunity. The chemokine receptor CCR5 has been implicated in the recruitment of Treg from blood into CRC and tumour growth is delayed in CCR5-/- mice, associated with reduced tumour Treg infiltration.

Methods

Tissue and blood samples were obtained from patients undergoing resection of CRC. Tumour-infiltrating lymphocytes were phenotyped for chemokine receptors using flow cytometry. The presence of tissue chemokines was assessed. Standard chemotaxis and suppression assays were performed and the effects of CCR5 blockade were tested in murine tumour models.

Results

Functional CCR5 was highly expressed by human CRC infiltrating Treg and CCR5(high) Treg were m

SUBMITTER: Ward ST 

PROVIDER: S-EPMC4301825 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature