Western white pine SNP discovery and high-throughput genotyping for breeding and conservation applications.

ABSTRACT:

Background

Western white pine (WWP, Pinus monticola Douglas ex D. Don) is of high interest in forest breeding and conservation because of its high susceptibility to the invasive disease white pine blister rust (WPBR, caused by the fungus Cronartium ribicola J. C. Fisch). However, WWP lacks genomic resource development and is evolutionarily far away from plants with available draft genome sequences. Here we report a single nucleotide polymorphism (SNP) study by bulked segregation-based RNA-Seq analysis.

Results

A collection of resistance germplasm was used for construction of cDNA libraries and SNP genotyping. Approximately 36-89 million 2 × 100-bp reads were obtained per library and de-novo assembly generated the first shoot-tip reference transcriptome containing a total of

SUBMITTER: Liu JJ 

PROVIDER: S-EPMC4302426 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature