Repurposing an endogenous degradation system for rapid and targeted depletion of C. elegans proteins.

ABSTRACT: The capability to conditionally inactivate gene function is essential for understanding the molecular basis of development. In gene and mRNA targeting approaches, protein products can perdure, complicating genetic analysis. Current methods for selective protein degradation require drug treatment or take hours for protein removal, limiting their utility in studying rapid developmental processes in vivo. Here, we repurpose an endogenous protein degradation system to rapidly remove targeted C. elegans proteins. We show that upon expression of the E3 ubiquitin ligase substrate-recognition subunit ZIF-1, proteins tagged with the ZF1 zinc-finger domain can be quickly degraded in all somatic cell types examined with temporal and spatial control. We demonstrate that genes can be engineered to beco

SUBMITTER: Armenti ST 

PROVIDER: S-EPMC4302935 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature