MiR-183/-96/-182 cluster is up-regulated in most breast cancers and increases cell proliferation and migration.

ABSTRACT:

Introduction

The miR-183/-96/-182 cluster is a conserved polycistronic microRNA (miRNA) cluster which is highly expressed in most breast cancers. Although there are some sporadic reports which demonstrate the importance of each miRNA in this cluster in breast cancer, the biological roles of this cluster as a whole and its regulation mechanisms in breast cancer are still unclear. We compared the expression of this cluster in different cancer types, analyzed the regulation mechanism of this cluster, identified new target genes, and examined the impact of this cluster on breast cancer cells.

Methods

The miRNA level was detected by LNA-based northern blot and Real-time PCR, and was also analyzed from TCGA dataset. Bioinformatics research and luciferase assay were applied to find

SUBMITTER: Li P 

PROVIDER: S-EPMC4303194 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature