Intracellular activation of trypsinogen in transgenic mice induces acute but not chronic pancreatitis.

ABSTRACT:

Background and aims

Premature intra-acinar activation of trypsinogen is widely considered key for both the initiation of acute pancreatitis and the development of chronic pancreatitis. However, the biological consequences of intracellular trypsinogen activation have not been directly examined. To do so, a new mouse model was developed.

Methods

Mice were engineered to conditionally express an endogenously activated trypsinogen within pancreatic acinar cells (PACE-tryp(on)). Hallmarks of pancreatitis were determined and findings were correlated to the level (zygosity) and extent (temporal and spatial) of conditional PACE-tryp(on) expression. Furthermore, the impact of acinar cell death in PACE-tryp(on) mice was assessed and compared with a model of selective diphtheria toxin (

SUBMITTER: Gaiser S 

PROVIDER: S-EPMC4304390 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature