Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening.

ABSTRACT: Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s(-1)) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same che

SUBMITTER: Cuttitta CM 

PROVIDER: S-EPMC4304690 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature