A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.

ABSTRACT: The lipid cubic phase or in meso method is a robust approach for crystallizing membrane proteins for structure determination. The uptake of the method is such that it is experiencing what can only be described as explosive growth. This timely, comprehensive and up-to-date review introduces the reader to the practice of in meso crystallogenesis, to the associated challenges and to their solutions. A model of how crystallization comes about mechanistically is presented for a more rational approach to crystallization. The possible involvement of the lamellar and inverted hexagonal phases in crystallogenesis and the application of the method to water-soluble, monotopic and lipid-anchored proteins are addressed. How to set up trials manually and automatically with a robot is introduced with ref

SUBMITTER: Caffrey M 

PROVIDER: S-EPMC4304740 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature