Efficient generation of recombinant influenza A viruses employing a new approach to overcome the genetic instability of HA segments.

ABSTRACT: Influenza A viruses (IAVs) are the most relevant and continual source of severe infectious respiratory complications in humans and different animal species, especially poultry. Therefore, an efficient vaccination that elicits protective and neutralizing antibodies against the viral hemagglutinin (HA) and neuraminidase (NA) is an important strategy to counter annual epidemics or occasional pandemics. With the help of plasmid-based reverse genetics technology, it is possible that IAV vaccine strains (IVVS) are rapidly generated. However, the genetic instability of some cloned HA-cDNAs after transformation into competent bacteria represents a major obstacle. Herein, we report efficient cloning strategies of different genetically volatile HA segments (H5- and H9-subtypes) employing either a ne

SUBMITTER: Mostafa A 

PROVIDER: S-EPMC4304806 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature