Interrogation of a context-specific transcription factor network identifies novel regulators of pluripotency.

ABSTRACT: The predominant view of pluripotency regulation proposes a stable ground state with coordinated expression of key transcription factors (TFs) that prohibit differentiation. Another perspective suggests a more complexly regulated state involving competition between multiple lineage-specifying TFs that define pluripotency. These contrasting views were developed from extensive analyses of TFs in pluripotent cells in vitro. An experimentally validated, genome-wide repertoire of the regulatory interactions that control pluripotency within the in vivo cellular contexts is yet to be developed. To address this limitation, we assembled a TF interactome of adult human male germ cell tumors (GCTs) using the Algorithm for the Accurate Reconstruction of Cellular Pathways (ARACNe) to analyze gene expres

SUBMITTER: Kushwaha R 

PROVIDER: S-EPMC4305010 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature