STK33 overexpression in hypopharyngeal squamous cell carcinoma: possible role in tumorigenesis.

ABSTRACT:

Background

The role of serine/threonine kinase 33 (STK33) gene in tumorigenesis is still controversial. This study was aimed to investigate whether STK33 had the effect on hypopharyngeal squamous cell carcinoma (HSCC) and relevant genes, as well as the potential relation to ERK1/2 pathway.

Methods

Immunohistochemistry was performed to investigate STK33 expression in human HSCC specimens. MTT, immunofluorescence, clone formation and matrigel invasion assays were employed to detect the effects of STK33 knockdown (STK33-RNAi) and/or PD98059 on major oncogenic properties of a HSCC cell line (Fadu), while, real-time PCR and western blot were used to examine the expressions of relevant genes.

Results

STK33 was over-expressed in HSCC specimens, which was significantly associ

SUBMITTER: Huang L 

PROVIDER: S-EPMC4305229 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature