Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria.

ABSTRACT:

Background

Both Dirofilaria repens and recently D. immitis are known to be endemic in Hungary. As one of several recent cases, the fatal case of a dog infested with D. immitis in Szeged, Southern Hungary, received attention from the media. Hence it was decided to catch mosquitoes in the garden where the dog lived to screen for filarioid helminths and Plasmodium spp. using molecular tools.

Methods

Mosquitoes were caught in Szeged, in the garden where the infected dog was kept, in July 2013 with M-360 electric mosquito traps and were stored in ethanol until further procedure. Female mosquitoes were classified to genus level by morphology. Each mosquito was homogenized and analyzed for filarioid helminths and avian malaria using standardized PCR techniques. Positive mosquito sa

SUBMITTER: Zittra C 

PROVIDER: S-EPMC4305256 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature