RNA-Seq reveals a role for NFAT-signaling in human retinal microvascular endothelial cells treated with TNFα.

ABSTRACT: TNFα has been identified as playing an important role in pathologic complications associated with diabetic retinopathy and retinal inflammation, such as retinal leukostasis. However, the transcriptional effects of TNFα on retinal microvascular endothelial cells and the different signaling pathways involved are not yet fully understood. In the present study, RNA-seq was used to profile the transcriptome of human retinal microvascular endothelial cells (HRMEC) treated for 4 hours with TNFα in the presence or absence of the NFAT-specific inhibitor INCA-6, in order to gain insight into the specific effects of TNFα on RMEC and identify any involvement of NFAT signaling. Differential expression analysis revealed that TNFα treatment significantly upregulated the expression of 579 genes when compa

SUBMITTER: Savage SR 

PROVIDER: S-EPMC4305319 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature