Development of an enhanced human gastrointestinal epithelial culture system to facilitate patient-based assays.

ABSTRACT:

Objective

The technology for the growth of human intestinal epithelial cells is rapidly progressing. An exciting possibility is that this system could serve as a platform for individualised medicine and research. However, to achieve this goal, human epithelial culture must be enhanced so that biopsies from individuals can be used to reproducibly generate cell lines in a short time frame so that multiple, functional assays can be performed (ie, barrier function and host-microbial interactions).

Design

We created a large panel of human gastrointestinal epithelial cell lines (n=65) from patient biopsies taken during routine upper and lower endoscopy procedures. Proliferative stem/progenitor cells were rapidly expanded using a high concentration of conditioned media containing t

SUBMITTER: VanDussen KL 

PROVIDER: S-EPMC4305344 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature