Multigene tracking of quasispecies in viral persistence and clearance of hepatitis C virus.

ABSTRACT:

Aim

To investigate the evaluation of hepatitis C virus (HCV) quasispecies in the envelope region and its relationship with the outcome of acute hepatitis C.

Methods

HCV quasispecies were characterized in specimens collected every 2-6 mo from a cohort of acutely HCV-infected subjects. We evaluated two individuals who spontaneously cleared viremia and three individuals with persistent viremia by cloning 33 1-kb amplicons that spanned E1 and the 5' half of E2, including hypervariable region 1 (HVR1). To assess the quasispecies complexity and to detect variants for sequencing, 33 cloned cDNAs representing each specimen were assessed by a combined method of analysis of a single-stranded conformational polymorphism and heteroduplex analysis. The rates of both synonymous and nonsyn

SUBMITTER: Chen S 

PROVIDER: S-EPMC4305653 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature