Identify lymphatic metastasis-associated genes in mouse hepatocarcinoma cell lines using gene chip.

ABSTRACT:

Aim

In order to obtain lymphogenous metastasis-associated genes, we compared the transcriptional profiles of mouse hepatocarcinoma cell lines Hca-F with highly lymphatic metastasis potential and Hca-P with low lymphatic metastasis potential.

Methods

Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA. In vitro transcription double-stranded cDNA was labeled with biotin (i.e. biotin-labeled cRNA, used as the probe). The cRNA probes hybridized with Affymetrix GeneChip(r) MOE430A (containing 22 690 transcripts, including 14 500 known mouse genes and 4 371 ESTs) respectively and the signals were scanned by the GeneArray Scanner. The results were then analyzed by bioinformatics.

Results

Out of the 14 500 known genes investigated, 110

SUBMITTER: Song B 

PROVIDER: S-EPMC4305688 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature