Gene expression profiling reveals sequential changes in gastric tubular adenoma and carcinoma in situ.

ABSTRACT:

Aim

To analyze the expression profiles of premalignant and/or preclinical lesions of gastric cancers.

Methods

We analyzed the expression profiles of normal gastric pit, tubular adenoma and carcinoma in situ using microdissected cells from routine gastric biopsies. For the DNA microarray analysis of formalin-fixed samples, we developed a simple and reproducible RNA extraction and linear amplification procedure applying two polymerase-binding sites. The amplification procedure took only 8 h and yielded comparable DNA microarray data between formalin-fixed tissues and unfixed controls.

Results

In comparison with normal pit, adenoma/carcinoma showed 504 up-regulated and 29 down-regulated genes at the expected false significance rate 0.15%. The differential expression betw

SUBMITTER: Lee CH 

PROVIDER: S-EPMC4305714 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature