Fluorescence endoscopy of cathepsin activity discriminates dysplasia from colitis.

ABSTRACT:

Background

Surveillance colonoscopy using random biopsies to detect colitis-associated cancer (CAC) suffers from poor sensitivity. Although chromoendoscopy improves detection, acceptance in the community has been slow. Here, we examine the usefulness of near infrared fluorescence (NIRF) endoscopy to image molecular probes for cathepsin activity in colitis-induced dysplasia.

Methods

In patient samples, cathepsin activity was correlated with colitis and dysplasia. In mice, cathepsin activity was detected as fluorescent hydrolysis product of substrate-based probes after injection into Il10(-/-) colitic mice. Fluorescence colonoscopy and colonic whole-mount imaging were performed before complete sectioning and pathology review of resected colons.

Results

Cathepsin activit

SUBMITTER: Gounaris E 

PROVIDER: S-EPMC4306589 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature