Designed surface residue substitutions in [NiFe] hydrogenase that improve electron transfer characteristics.

ABSTRACT: Photobiological hydrogen production is an attractive, carbon-neutral means to convert solar energy to hydrogen. We build on previous research improving the Alteromonas macleodii "Deep Ecotype" [NiFe] hydrogenase, and report progress towards creating an artificial electron transfer pathway to supply the hydrogenase with electrons necessary for hydrogen production. Ferredoxin is the first soluble electron transfer mediator to receive high-energy electrons from photosystem I, and bears an electron with sufficient potential to efficiently reduce protons. Thus, we engineered a hydrogenase-ferredoxin fusion that also contained several other modifications. In addition to the C-terminal ferredoxin fusion, we truncated the C-terminus of the hydrogenase small subunit, identified as the available ter

SUBMITTER: Yonemoto IT 

PROVIDER: S-EPMC4307346 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature