A GSK-3β inhibitor protects against radiation necrosis in mouse brain.

ABSTRACT:

Purpose

To quantify the effectiveness of SB415286, a specific inhibitor of GSK-3β, as a neuroprotectant against radiation-induced central nervous system (brain) necrosis in a mouse model.

Methods and materials

Cohorts of mice were treated with SB415286 or dimethyl sulfoxide (DMSO) prior to irradiation with a single 45-Gy fraction targeted to the left hemisphere (brain) using a gamma knife machine. The onset and progression of radiation necrosis (RN) were monitored longitudinally by noninvasive in vivo small-animal magnetic resonance imaging (MRI) beginning 13 weeks postirradiation. MRI-derived necrotic volumes for SB415286- and DMSO-treated mice were compared. MRI results were supported by correlative histology.

Results

Mice treated with SB415286 showed significant pr

SUBMITTER: Jiang X 

PROVIDER: S-EPMC4307920 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature