Evaluation of methods to purify virus-like particles for metagenomic sequencing of intestinal viromes.

ABSTRACT:

Background

Viruses are a significant component of the intestinal microbiota in mammals. In recent years, advances in sequencing technologies and data analysis techniques have enabled detailed metagenomic studies investigating intestinal viromes (collections of bacteriophage and eukaryotic viral nucleic acids) and their potential contributions to the ecology of the microbiota. An important component of virome studies is the isolation and purification of virus-like particles (VLPs) from intestinal contents or feces. Several methods have been applied to isolate VLPs from intestinal samples, yet to our knowledge, the efficiency and reproducibility between methods have not been explored. A rigorous evaluation of methods for VLP purification is critical as many studies begin to move from

SUBMITTER: Kleiner M 

PROVIDER: S-EPMC4308010 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature