Discovery of epigenetic biomarkers for the noninvasive diagnosis of fetal disease.

ABSTRACT:

Objectives

The primary goal of this study was to identify CpG sites in the human genome that are differentially methylated in DNA obtained from chorionic villus sampling (CVS) samples and gestational age-matched maternal blood cell (MBC) samples.

Methods

We used the HumanMethylation27 DNA Analysis BeadChip to characterize DNA methylation in samples of CVS and MBC. We then selected a subset of differentially methylated CpG sites on chromsome 13 and subjected them to analysis by mass spectrometry using the Epityper platform.

Results

We identified 718 tissue-specific differentially methylated regions (DMRs) between MBC and CVS; 563 of these were hypermethylated in MBC and hypomethylated in CVS, whereas 155 sites were hypomethylated in MBC and hypermethylated in CVS. Furt

SUBMITTER: Bunce K 

PROVIDER: S-EPMC4308692 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature