In silico identification of microRNAs predicted to regulate the drug metabolizing cytochrome P450 genes.

ABSTRACT:

Objective

Cytochrome P450 (CYP) enzymes exhibit high interindividual variability that is not completely explained by known environmental and genetic factors. To further understand this variability, we hypothesized that microRNAs (miRNAs) may regulate CYP expression.

Methods

MiRNA identification algorithms were used to identify the miRNAs that are predicted to regulate twelve major drug metabolizing CYPs and to identify polymorphisms in CYP mRNA 3'-UTRs that are predicted to interfere with normal mRNA-miRNA interactions.

Results

All twelve CYPs were predicted to be targets of miRNAs. Additionally, 38 SNPs in CYP mRNA 3'-UTRs were predicted to interfere with miRNA targeting of mRNAs. These predicted miRNAs and SNPs are candidates for future in vitro studies focused on u

SUBMITTER: Ramamoorthy A 

PROVIDER: S-EPMC4308728 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature