Embryonic cells contribute directly to the quiescent stem cell population in the adult mouse mammary gland.

ABSTRACT:

Introduction

Studies have identified multi-potent stem cells in the adult mammary gland. More recent studies have suggested that the embryonic mammary gland may also contain stem/progenitor cells that contribute to initial ductal development. We were interested in determining whether embryonic cells might also directly contribute to long-lived stem cells that support homeostasis and development in the adult mammary gland.

Methods

We used DNA-label retention to detect long label-retaining cells in the mammary gland. Mouse embryos were labeled with 5-ethynl-2'-deoxyuridine (EdU) between embryonic day 14.5 and embryonic day 18.5 and were subsequently sacrificed and examined for EdU retention at various intervals after birth. EdU retaining cells were co-stained for various linea

SUBMITTER: Boras-Granic K 

PROVIDER: S-EPMC4308878 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature