DNA methylation is developmentally regulated for genes essential for cardiogenesis.

ABSTRACT:

Background

DNA methylation is a major epigenetic mechanism altering gene expression in development and disease. However, its role in the regulation of gene expression during heart development is incompletely understood. The aim of this study is to reveal DNA methylation in mouse embryonic hearts and its role in regulating gene expression during heart development.

Methods and results

We performed the genome-wide DNA methylation profiling of mouse embryonic hearts using methyl-sensitive, tiny fragment enrichment/massively parallel sequencing to determine methylation levels at ACGT sites. The results showed that while global methylation of 1.64 million ACGT sites in developing hearts remains stable between embryonic day (E) 11.5 and E14.5, a small fraction (2901) of them exhibi

SUBMITTER: Chamberlain AA 

PROVIDER: S-EPMC4309105 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature