The demonstration of αKlotho deficiency in human chronic kidney disease with a novel synthetic antibody.

ABSTRACT:

Background

αKlotho is the prototypic member of the Klotho family and is most highly expressed in the kidney. αKlotho has pleiotropic biologic effects, and in the kidney, its actions include regulation of ion transport, cytoprotection, anti-oxidation and anti-fibrosis. In rodent models of chronic kidney disease (CKD), αKlotho deficiency has been shown to be an early biomarker as well as a pathogenic factor. The database for αKlotho in human CKD remains controversial even after years of study.

Methods

We used a synthetic antibody library to identify a high-affinity human antigen-binding fragment that recognizes human, rat and mouse αKlotho primarily in its native, rather than denatured, form.

Results

Using an immunoprecipitation-immunoblot (IP-IB) assay, we measured bot

SUBMITTER: Barker SL 

PROVIDER: S-EPMC4309192 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature