Identification of reference genes in human myelomonocytic cells for gene expression studies in altered gravity.

ABSTRACT: Gene expression studies are indispensable for investigation and elucidation of molecular mechanisms. For the process of normalization, reference genes ("housekeeping genes") are essential to verify gene expression analysis. Thus, it is assumed that these reference genes demonstrate similar expression levels over all experimental conditions. However, common recommendations about reference genes were established during 1 g conditions and therefore their applicability in studies with altered gravity has not been demonstrated yet. The microarray technology is frequently used to generate expression profiles under defined conditions and to determine the relative difference in expression levels between two or more different states. In our study, we searched for potential reference genes with stab

SUBMITTER: Thiel CS 

PROVIDER: S-EPMC4309215 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature