Capacitance-modulated transistor detects odorant binding protein chiral interactions.

ABSTRACT: Peripheral events in olfaction involve odorant binding proteins (OBPs) whose role in the recognition of different volatile chemicals is yet unclear. Here we report on the sensitive and quantitative measurement of the weak interactions associated with neutral enantiomers differentially binding to OBPs immobilized through a self-assembled monolayer to the gate of an organic bio-electronic transistor. The transduction is remarkably sensitive as the transistor output current is governed by the small capacitance of the protein layer undergoing minute changes as the ligand-protein complex is formed. Accurate determination of the free-energy balances and of the capacitance changes associated with the binding process allows derivation of the free-energy components as well as of the occurrence of c

SUBMITTER: Mulla MY 

PROVIDER: S-EPMC4309438 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature