A statistical analysis of murine incisional and excisional acute wound models.

ABSTRACT: Mice represent the most commonly used species for preclinical in vivo research. While incisional and excisional acute murine wound models are both frequently employed, there is little agreement on which model is optimum. Moreover, current lack of standardization of wounding procedure, analysis time point(s), method of assessment, and the use of individual wounds vs. individual animals as replicates makes it difficult to compare across studies. Here we have profiled secondary intention healing of incisional and excisional wounds within the same animal, assessing multiple parameters to determine the optimal methodology for future studies. We report that histology provides the least variable assessment of healing. Furthermore, histology alone (not planimetry) is able to detect accelerated hea

SUBMITTER: Ansell DM 

PROVIDER: S-EPMC4309476 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature