Cryopreservation of MHC multimers: Recommendations for quality assurance in detection of antigen specific T cells.

ABSTRACT: Fluorescence-labeled peptide-MHC class I multimers serve as ideal tools for the detection of antigen-specific T cells by flow cytometry, enabling functional and phenotypical characterization of specific T cells at the single cell level. While this technique offers a number of unique advantages, MHC multimer reagents can be difficult to handle in terms of stability and quality assurance. The stability of a given fluorescence-labeled MHC multimer complex depends on both the stability of the peptide-MHC complex itself and the stability of the fluorochrome. Consequently, stability is difficult to predict and long-term storage is generally not recommended. We investigated here the possibility of cryopreserving MHC multimers, both in-house produced and commercially available, using a wide range

SUBMITTER: Hadrup SR 

PROVIDER: S-EPMC4309491 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature