Dynamic migration and cell-cell interactions of early reprogramming revealed by high-resolution time-lapse imaging.

ABSTRACT: Discovery of the cellular and molecular mechanisms of induced pluripotency has been hampered by its low efficiency and slow kinetics. Here, we report an experimental system with multicolor time-lapse microscopy that permits direct observation of pluripotency induction at single cell resolution, with temporal intervals as short as 5 minutes. Using granulocyte-monocyte progenitors as source cells, we visualized nascent pluripotent cells that emerge from a hematopoietic state. We engineered a suite of image processing and analysis software to annotate the behaviors of the reprogramming cells, which revealed the highly dynamic cell-cell interactions associated with early reprogramming. We observed frequent cell migration, which can lead to sister colonies, satellite colonies, and colonies of m

SUBMITTER: Megyola CM 

PROVIDER: S-EPMC4309553 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature